Current Experimental Methods for Characterizing Protein-Protein Interactions
نویسندگان
چکیده
منابع مشابه
Characterizing Protein-Protein interactions by iTC
Proteins operate as integral members of complex networks that precisely regulate physiological processes through a combination of signaling pathways and feedback. These regulatory functions, including signal transduction, protein trafficking, transcription and translation, all rely on the ability of proteins to rapidly target and form specific non-covalent complexes with other proteins in respo...
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MOTIVATION Despite advances in high-throughput methods for discovering protein-protein interactions, the interaction networks of even well-studied model organisms are sketchy at best, highlighting the continued need for computational methods to help direct experimentalists in the search for novel interactions. RESULTS We present a kernel method for predicting protein-protein interactions usin...
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Protein-protein interactions (PPIs) play a critical role in many cellular functions. A number of experimental techniques have been applied to discover PPIs; however, these techniques are expensive in terms of time, money, and expertise. There are also large discrepancies between the PPI data collected by the same or different techniques in the same organism. We therefore turn to computational t...
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The hepatopancreas protein, therefore, probably represents the membranous proton channel of the vacuolar ATPase and electron microscopy has given us the clearest impression yet of its organization; an organization clearly consistent with the functional characteristics of the chloroplast ATPase-CF,, complex [22]. It is hoped that techniques such as electron diffraction may improve the structural...
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The increasing rate at which complete genome sequences become available necessitates rapid and robust methods for investigating the functions of their encoded proteins. Efforts have been made to study protein function by systematically screening large sets of proteins using the two-hybrid method. Analyses of the complete proteomes of baceriophage T7, the mammalian viruses hepatitis C and vaccin...
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ژورنال
عنوان ژورنال: ChemMedChem
سال: 2016
ISSN: 1860-7179
DOI: 10.1002/cmdc.201500495